Radiation Genes Help Page

What is Radiation Genes?

The Radiation Genes Database collects microarrays data on transcriptional effects of ionizing radiation obtained from public repositories or from published papers.
This database should allow researchers working on transcriptional response of mammalian cells to radiation, facilitating comparison and meta-clustering analysis.

How do I ask questions?

Please send an e-mail to tiziana.castrignano@caspur.it.

How can I use Radiation Genes?

Radiation Genes usage is absolutely free of charge.
The submission page allows registered members to submit or update their experiments.
A search page instead allows users to select criteria of their queries in order to check the collected experiments.

How can I submit experiments?

Before introducing experiments data, you should register an account in the Register page.
Registration is instant and simple, all you have to do is to fill in a form, as shown in figure 1:

Fig. 1 - Registration form

A validation message will confirm the correctness of the registration:

Fig. 2 - Registration confirmation

After registration, you can click on the submit button on the left menu and fill in the Login form.
Once logged in, the submit page becomes available (click here for a full view of it).
Before filling in the main submission form, it is possible to import values from an old experiment by clicking on the View your experiment list link.

Fig. 3 & 4 - Import Experiment

Click on an experiment, then press the Import button. All the experiment data will be loaded in the main submit form, as shown in figure 6:

Fig. 5 - Partial view of the Submit form. Click on image to open the full view

After filling in the form, click the Submit button to save the new experiment. If the experiment had been imported, it is possible to:
- overwrite the previous version by saving it with the old name
- save it as a new experiment by changing his name in the first input field (the Array design name).

How can I search experiments info?

In the search page users can easily query experiments specifiying their search parameters, such as Gene name, Time and Dose. Detailed informations about each value are available by clicking on the question mark help buttons.

Fig. 6 - Search form

Note that the selection of genes appearing within a fixed cut-off in a percentage of experiments is an advanced search function, as it requires slightly more time to calculate; thereby it is recommended to use this function only in a restricted set of results.
Click on the Search! button and a results table will be shown in the page, as in the next figure:

Fig. 7 - Results table. Click on image to open the full view

Click on the Show Heat Map button to open the Heat Map corresponding to the resultant genesi.

Fig. 8 - Heat Map Preview. Click on image to open the full view

Click on the Export button to save a tab-delimited file containing the search results.

Fig. 9 - Tab-delimited file preview. Click on image to open the full view